Performance and technical specifications

Intended use

AroCell TK 210 ELISA is intended for the measurement of Thymidine Kinase 1 (TK1) in human serum.

Regulatory approvals

AroCell TK 210 ELISA is CE-marked and compliant with the European Union IVD directive. The product is registered by the Swedish Medical Product Agency.

AroCell TK 210 ELISA is labeled For Research Use Only in the USA, China, S. Korea, Tunisia, Algeria, Morocco, Canada, India, and Mexico.

Technical specifications

Assay format: Microplate sandwich ELISA
Size: 1 × 96 wells
Calibration curve: 0–17 ng/mL
 Assay Time: 4.75 h
Sample Type: Human serum, Lithium Heparin plasma, cell culture extract, and cell culture supernatant.
Sample Size: 180 µL
Species: Human
Calibrator: Human recombinant TK1

Performance characteristics

Limited of detection: 0.12 ng/mL
Measuring range: 0.12 – 17 ng/mL
The range may be extended by increasing sample dilution.
Dilution recovery: <5% deviation from linearity.
Precision within assay: At TK1 conc. 1.85 ng/mL: CV% = 4.9%
At TK1 conc. 11.53 ng/mL: CV% = 1.9%
Precision between assay: At TK1 conc. 1.85 ng/mL: CV% = 0.9%
At TK1 conc. 11.53 ng/mL: CV% = 2.2%
Precision total: At TK1 conc. 1.85 ng/mL: CV% = 5.1%
At TK1 conc. 11.53 ng/mL: CV% = 3.0%
Specificity: No cross-reaction with TK2 or non-human TK1


Typical calibration curve obtained using AroCell TK 210 ELISA. The plot of A450nm versus [TK1] ng/mL. This is for illustration only. Do not use for the calculation of samples (ref. 31-11TF710B1-05).


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  9. Jagarlamudi, K.K. et al. Breast and Prostate Cancer Patients Differ Significantly in Their Serum Thymidine Kinase 1 (TK1) Specific Activities Compared with Those Found in Hematological Malignancies and Blood Donors: Implications of Using Serum TK1 as a Biomarker. BMC Cancer 15 (2015), 66.
  10. Kumar, J.K. et al. A Clinical Evaluation of the TK 210 ELISA in Sera from Breast Cancer Patients Demonstrates High Sensitivity and Specificity at All Stages of the Disease. Tumor Biology 37(9) (2016), 11937-11945.